GsMTx4 ndi peptide ya 35-amino acid yokhala ndi zomangira zinayi za disulfide zomwe zimapanga cysteine knot motif, yomwe ndi gawo lodziwika bwino la ma peptides ambiri a kangaude omwe amapereka kukhazikika komanso kutsimikizika.Njira ya GsMTx4 sinafotokozedwe bwino, koma imakhulupirira kuti imamangiriza ku madera a extracellular kapena transmembrane a cationic MSCs ndikutchinga kutseguka kwa pore kapena kutsekeka kwawo posintha mawonekedwe awo kapena kupsinjika kwa membrane.GsMTx4 yasonyezedwa kuti imalepheretsa ma MSC angapo a cationic ndi kusankha kosiyana ndi potency.Mwachitsanzo, GsMTx4 imaletsa TRPC1 yokhala ndi IC50 ya 0.5 μM, TRPC6 yokhala ndi IC50 ya 0.2 μM, Piezo1 yokhala ndi IC50 ya 0.8 μM, Piezo2 yokhala ndi IC50 ya 0.3 μM, koma ilibe mphamvu pa TRP40 mpaka TRP41 μM.(Bae C et al 2011, Biochemistry)
GsMTx4 yakhala ikugwiritsidwa ntchito ngati chida chamankhwala chophunzirira ntchito ndi kuwongolera kwa ma cationic MSCs mumitundu yosiyanasiyana yama cell ndi minofu.Zina mwa zitsanzo ndi:
GsMTx4 ikhoza kuletsa ma MSC omwe amayendetsedwa ndi kutambasula mu astrocyte, maselo a mtima, maselo osalala a minofu ndi maselo a minofu ya chigoba.Astrocyte ndi maselo ooneka ngati nyenyezi omwe amathandiza ubongo ndi msana.Maselo a mtima ndi maselo omwe amapanga minofu ya mtima.Maselo a minofu yosalala ndi maselo omwe amayendetsa kayendedwe ka ziwalo monga mimba ndi mitsempha ya magazi.Maselo a minofu ya chigoba ndi maselo omwe amathandiza kuyenda mwaufulu kwa thupi.Mwa kutsekereza ma MSC m'maselo awa, GsMTx4 imatha kusintha mphamvu zawo zamagetsi, kuchuluka kwa calcium, kutsika ndi kumasuka, komanso mawonekedwe a jini.Zosinthazi zimatha kukhudza momwe maselowa amagwirira ntchito moyenera kapena m'matenda (Suchyna et al., Nature 2004; Bae et al., Biochemistry 2011; Ranade et al., Neuron 2015; Xiao et al., Nature Chemical Biology 2011)
GsMTx4 imathanso kuletsa mtundu wapadera wa MSC wotchedwa TACAN, womwe umakhudzidwa ndi kuyankha kwa ululu.TACAN ndi njira yomwe imawonekera m'maselo a mitsempha omwe amamva kupweteka.TACAN imayendetsedwa ndi zokopa zamakina, monga kukakamiza kapena kukanikiza, ndipo zimayambitsa zowawa.GsMTx4 ikhoza kuchepetsa ntchito ya TACAN ndi kuchepetsa khalidwe la ululu mu zinyama za ululu wamakina (Wetzel et al., Nature Neuroscience 2007; Eijkelkamp et al., Nature Communications 2013)
GsMTx4 imatha kuteteza ma astrocyte ku kawopsedwe koyambitsidwa ndi molekyulu yotchedwa lysophosphatidylcholine (LPC), yomwe ndi mkhalapakati wamafuta omwe amawononga ubongo ndi msana.LPC imatha kuyambitsa ma MSC mu astrocyte ndikupangitsa kuti atenge kashiamu wochulukirapo, zomwe zimabweretsa kupsinjika kwa okosijeni ndi kufa kwa cell.GsMTx4 ingalepheretse LPC kuyambitsa ma MSC mu astrocyte ndikuwateteza ku kawopsedwe.GsMTx4 imathanso kuchepetsa kuwonongeka kwaubongo ndikuwongolera magwiridwe antchito a minyewa mu mbewa zomwe zidabayidwa ndi LPC (Gottlieb et al., Journal of Biological Chemistry 2008; Zhang et al., Journal of Neurochemistry 2019)
GsMTx4 imatha kusintha kusiyana kwa maselo a neural stem cell poletsa mtundu wina wa MSC wotchedwa Piezo1, womwe umawonetsedwa m'maselo a neural stem.Maselo a Neural stem ndi maselo omwe amatha kupanga ma neuron atsopano kapena mitundu ina ya ma cell aubongo.Piezo1 ndi njira yomwe imayendetsedwa ndi njira zamakina zochokera ku chilengedwe, monga kuuma kapena kupanikizika, ndipo zimakhudza momwe maselo a neural stem amasankha mtundu wa selo kukhala.GsMTx4 ikhoza kusokoneza ntchito ya Piezo1 ndikusintha kusiyana kwa maselo a neural stem kuchokera ku neurons kupita ku astrocyte (Pathak et al., Journal of Cell Science 2014; Lou et al., Cell Reports 2016)
Ndife opanga ma polypeptide ku China, omwe ali ndi zaka zingapo okhwima pakupanga polypeptide.Hangzhou Taijia Biotech Co., Ltd. ndi katswiri wopanga polypeptide yaiwisi, yomwe imatha kupereka makumi masauzande azinthu zopangira polypeptide ndipo imathanso kusinthidwa malinga ndi zosowa.Ubwino wa zinthu za polypeptide ndi zabwino kwambiri, ndipo chiyero chimatha kufika 98%, chomwe chadziwika ndi ogwiritsa ntchito padziko lonse lapansi.
