Pepstatin ndi pentapeptide, mwachilengedwe aspartyl protease inhibitor, yomwe imatha kuletsa aspartic protease ndi acid protease yamitundu yosiyanasiyana.Pepstatin imapangidwa makamaka ndi mitundu ya Streptomyces ndipo imapangidwa ndi Streptomyces.Ikhoza kuletsa pepsin, pepsin D ndi angiotensin-kumasula enzyme, ndipo ali ndi achire zotsatira pa chapamimba chilonda, aimpso matenda oopsa, nyamakazi, carrageenan edema ndi matenda ena.
Pepstatin ndi inhibitor yamphamvu ya aspartyl proteases monga pepsin, cathepsin D, ndi renin.Pentapeptide yachilengedwe iyi yomwe idasiyanitsidwa ndi actinomycetes inali kwa zaka zambiri renin inhibitor in vitro.Pepstatin sikutanthauza renin ndipo imasungunuka bwino m'madzi.Pepstatin ili ndi γ amino acid statin yachilendo yomwe ingalowe m'malo mwa ma amino acid awiri pagawo la puloteni ndikutsekeka kwa gawo lapansi chifukwa cha kufanana kwake. ku kusintha kwa peptide bond hydrolysis ndi aspartyl proteases.
Mapuloteni akachotsedwa m’maselo osweka, ma protease amatha kutulutsidwa, omwe amafunika kuletsedwa mofulumira kuti mapuloteni asawonongeke.Pochotsa mapuloteni, ma protease inhibitors amafunika kuwonjezeredwa kuti ateteze proteinolysis.Protease inhibitor imatanthawuza mozama za chinthu chomwe chimamangiriza kumagulu ena pakatikati pa mamolekyu a protease, kotero kuti ntchito ya protease imachepa kapena kutha, koma osasintha mapuloteni a enzyme.The tilinazo zosiyanasiyana proteases osiyana mapuloteni ndi osiyana, choncho m`pofunika kusintha ndende zosiyanasiyana proteases.Chifukwa kusungunuka kwa protease inhibitor mu zamadzimadzi ndikotsika kwambiri, ziyenera kuperekedwa chidwi chapadera kuti protease inhibitor iyenera kusakanizidwa bwino mu nkhokwe kuti achepetse mvula ya protease inhibitor.Pepstantin A imatha kuletsa ma protease a asidi monga pepsin, angiotensin, collagenase, cathepsin D ndi chymosin.
Pepstatin A ndi inhibitor ya cathepsin d ndi e.Pambuyo pa ma cell a HEK293 adathandizidwa ndi ma pepstatin A osiyanasiyana kwa maola 24, mawu a LC3Ⅱ ndi p62 adapezeka.Zotsatira zake zidawonetsa kuti pepstatin A imatha kupititsa patsogolo kufotokozera kwa LC3Ⅱ ndi P62 (P<0.05) m'njira yodalira mlingo.20μ g/ml pepstatin A idagwiritsidwa ntchito pochiza HEK293 pazigawo zosiyanasiyana zanthawi, ndipo zotsatira za nthawi zosiyanasiyana pamawu a LC3II ndi p62 zidawonedwa.Zotsatira zake zidawonetsa kuti pepstatin A imatha kuwongolera mawu a LC3II ndi p62 motengera nthawi.
Ndife opanga ma polypeptide ku China, omwe ali ndi zaka zingapo okhwima pakupanga polypeptide.Hangzhou Taijia Biotech Co., Ltd. ndi katswiri wopanga polypeptide yaiwisi, yomwe imatha kupereka makumi masauzande azinthu zopangira polypeptide ndipo imathanso kusinthidwa malinga ndi zosowa.Ubwino wa zinthu za polypeptide ndi zabwino kwambiri, ndipo chiyero chimatha kufika 98%, chomwe chadziwika ndi ogwiritsa ntchito padziko lonse lapansi.
